Sequence data of mice transcriptome (2019)

According to the genomic data obtained, most of the candidate genes are related to physiological development. In order to study the specific regulatory mechanism of the candidate genes, corresponding functional verification tests are carried out. Therefore, we obtained the corresponding transgenic mice and sequenced the corresponding tissues of homozygous and wild-type samples (22 tissue samples in total, including brain, bone marrow and muscle tissues). Then through the analysis of this batch of transcriptome data, we can improve the functional verification of candidate genes, in order to provide powerful data for understanding the adaptive genetic mechanism of species in different regions and physiological regulation in the process of growth and development.

0 2020-08-18

Y chromosome SNP and STR data of Tibetans from Lhasa

To investigate the paternal genetic structure of Tibetans from Lhasa, 1029 male samples were collected from Lhasa, Tibet. Firstly, SNP genotyping was performed to allocate samples into haplogroups. To further evaluate the genetic diversity of the major Y-chromosomal haplogroup in Tibetan populations from Lhasa, eight commonly used Y-chromosomal STR (short tandem repeat) loci (DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393) were genotyped using fluorescence-labeled primers with an ABI 3130XL Genetic Analyzer (Applied Biosystems, USA). The results indicated that haplogroup D-M174 displayed highest frequency in Lhasa Tibetans (56.56%, the majority of its sublineages were D3*-P99), followed by haplogroups O-M175 (30.71%, with most of the samples belonging to O3a3c1-M117). Another relatively rare lineages in Lhasa Tibetans were N-M231 (5.15%, especially its sublineage N1*-LLY22G), C-M130 (2.62%), R-M207 (2.53%), Q (1.55%), J (0.68%), K-M, and T. Further analysis indicated that the Lhasa Tibetans’ Y chromosome haplogroups have ages within different periods, including >30 kya, LGM, post-LGM, Holocene, indicating occupation of modern humans in different periods.

0 2020-03-19