一. Data overview This data interchange is the second data interchange of "genomics research on drought tolerance mechanism of typical desert plants in heihe basin", a key project of the major research program of "integrated research on eco-hydrological processes in heihe basin".The main research goal of this project is a typical desert sand Holly plants as materials, using the current international advanced a new generation of gene sequencing technology to the whole genome sequence and gene transcription of Holly group sequence decoding, so as to explore related to drought resistance gene and gene groups, and transgenic technology in model plants such as arabidopsis and rice) verify its drought resistance. 二, data content 1.Sequencing of the genome and transcriptome of lycophylla SPP. The genome size of Mongolian Holly was about 926 Mb, GC content 36.88%, repeat sequence proportion 66%, genome heterozygosity rate 0.56%, which indicated that the genome has many repeat sequences, high heterozygosity and belongs to a complex genome.Based on the predicted sequence results, we subsequently carried out in-depth sequencing of the genome of lysiopsis SPP. The obtained data were assembled to obtain a 937 Mb genome sequence (table 1), which was basically the same as the predicted genome size.Through to the sand Holly transcriptome sequencing and sequence assembly (table 2), received more than 77000 genes coding sequence (Unigene), these sequences are comments found that most of the gene sequence and legumes and soybean, garbanzo beans and bean has a higher similarity (figure 1), consistent with the fact of sand ilex leguminous plants. 一), and the sand Holly is a leguminous plants consistent with the fact. 2.Discovery of simple repeat sequence (SSR) molecular markers of sand Holly: There is a transcriptome data set of sand Holly in the network public database, and the sample collection site is zhongwei city, ningxia.But this is the location of the project team samples in minqin county, gansu province, in order to study whether this sand in different areas of the Holly sequence has sequence polymorphism, we first identify the minqin county plant samples in the genomes of simple sequence repeat (SSR) markers (table 3), and then, compares the transcriptome sequences of plant sample, found in part of SSR molecular marker polymorphism (table 4), these molecular markers could be used for the species of plant genetic map construction, QTL mapping and genetic diversity analysis in the study. 三, data processing instructions Sample collection place: minqin county, gansu province, latitude and longitude: N38 ° 34 '25.93 "E103 ° 08' 36.77".Genome sequencing: a total of 8 genomic DNA libraries of different sizes were constructed and determined by Illumina HiSeq 2500 instrument.Transcriptome sequencing: a library of 24 transcriptome mrnas was constructed and determined by Illumina HiSeq 4000. 四, the use of data and meaning We selected a typical desert plant as the research object, from the Angle of genomics, parse the desert plant genome and transcriptome sequences, excavated its precious drought-resistant gene resources, and to study their drought resistance mechanism of favorable sand Holly this ancient and important to the utilization of plant resources, as well as the heihe river basin of drought-resistant plant genetic breeding, ecological restoration and sustainable development.
一. data description The data included the precipitation, river water and groundwater in the small calabash valley from July to September 2015 2H, 18O, with a sampling frequency of 2 weeks/time. 二. Sampling location (1) the precipitation sampling point is located in the ecological hydrology station of the institute of cold and dry regions, Chinese academy of sciences, with the latitude and longitude of 99 ° 53 '06.66 "E, 38 ° 16' 18.35" N. (2) the sampling point of the river is located at the outlet flow weir of haugugou small watershed in the upper reaches of the heihe river, with the latitude and longitude of 99 ° 52 '47.7 "E and 38 ° 16' 11" N.The water sampling point number 2 position for heihe river upstream hoist ditch Ⅱ area exports, latitude and longitude 99 ° 52 '58.40 "E, 38 ° 14' 36.85" N. (3) underground water spring and well water sampling points.The sampling point of spring water is located at 20m to the east of the outlet of the basin, with the latitude and longitude of 99°52 '50.9 "E, 38°16' 11.44" N. The well water sampling point is located near the intersection of east and west branches, with the latitude and longitude of 99 ° 52 '45.38 "E, 38 ° 15' 21.27" N. 三. Test method The δ2H and δ18O values of the samples were measured by PICARRO L2130-i ultra-high precision liquid water and water vapor isotope analyzer. The results were expressed by the test accuracy value of v-smow relative to the international standard substance, and the measurement accuracy was 0.038‰ and 0.011‰, respectively.
1、 Data Description: the data includes the samples of anions and anions of river water and groundwater in hulugou small watershed from July to September 2015 for test and analysis. The sampling frequency is once every two weeks. 2、 Sampling location: (1) there are two river water sampling points. One is located at the outlet flow weir of hulugou small watershed in the upper reaches of Heihe River, with latitude and longitude of 99 ° 52 ′ 47.7 ″ E and 38 ° 16 ′ 11 ″ n. The second sampling point of the river is located at the outlet of hulugou area II at the upper reaches of Heihe River, with the longitude and latitude of 99 ° 52 ′ 58.40 ″ E and 38 ° 14 ′ 36.85 ″ n. (2) Underground water spring and well water sampling points are 20 m to the east of the drainage basin outlet, with longitude and latitude of 99 ° 52 ′ 50.9 ″ E and 38 ° 16 ′ 11.44 ″ n. The well water sampling point is located near the intersection of the East and West Branch ditches, with the longitude and latitude of 99 ° 52 ′ 45.38 ″ E and 38 ° 15 ′ 21.27 ″ n. 3、 Test method: the cation of sample is tested by inductively coupled plasma atomic emission spectrometer (ICP-AES), the test accuracy is 0.05mg/l, and the anion is tested by ion chromatograph (ics1100), the test accuracy is 0.002mg/l.
1、 Data Description: data includes doc and DIC values of river water and groundwater in hulugou small watershed from July to September 2015. The sampling frequency is once every two weeks. 2、 Sampling location: (1) there are two river water sampling points. The first sampling point is located at the hydrological section at the outlet of hulugou Small Watershed at the upper reaches of Heihe River, with the longitude and latitude of 99 ° 52 ′ 47.7 ″ E and 38 ° 16 ′ 11 ″ n. The second sampling point of the river is located at the outlet of hulugou area II at the upper reaches of Heihe River, with the longitude and latitude of 99 ° 52 ′ 58.40 ″ E and 38 ° 14 ′ 36.85 ″ n. (2) Underground water spring and well water sampling points. The spring sampling point is located at 20 m to the east of the drainage basin outlet, with the longitude and latitude of 99 ° 52 ′ 50.9 ″ E and 38 ° 16 ′ 11.44 ″ n. The well water sampling point is located near the intersection of the East and West Branch ditches, with the longitude and latitude of 99 ° 52 ′ 45.38 ″ E and 38 ° 15 ′ 21.27 ″ n. 3、 Test method: Doc and DIC values of samples were measured by oiaurora 1030w TOC instrument, detection range: 2ppb c-30000ppm C.
The data set contains NPP products data produced by the maximum synthesis method of the three source regions of the Yellow River, the Yangtze River and the Lancang River. The data of remote sensing products MOD13Q1, MOD17A2, and MOD17A2H are available on the NASA website (http://modis.gsfc.nasa.gov/). The MOD13Q1 product is a 16-d synthetic product with a resolution of 250 m. The MOD17A2 and MOD17A2H product data are 8-d synthetic products, the resolution of MOD17A2 is 1 000 m, and the resolution of MOD17A2H is 500 m. The final synthetic NPP product of MODIS has a resolution of 1 km. The downloaded MOD13Q1, MOD17A2, and MOD17A2H remote sensing data products are in HDF format. The data have been processed by atmospheric correction, radiation correction, geometric correction, and cloud removal. 1) MRT projection conversion. Convert the format and projection of the downloaded data product, convert the HDF format to TIFF format, convert the projection to the UTM projection, and output NDVI with a resolution of 250 m, EVI with a resolution 250 m, and PSNnet with resolutions of 1 000 m and 500 m. 2) MVC maximum synthesis. Synthesize NDVI, EVI, and PSNnet synchronized with the ground measured data by the maximum value to obtain values corresponding to the measured data. The maximum synthesis method can effectively reduce the effects of clouds, the atmosphere, and solar elevation angles. 3) NPP annual value generated from the NASA-CASA model.
This dataset includes the temperature, precipitation, relative humidity, wind speed, wind direction and other daily values in the observation point of Shiquan River Source. The data is observed from July 2, 2012 to August 5, 2014, and from September 30, 2015 to December 25, 2015. It is measured by automatic meteorological station (Onset Company) and a piece of data is recorded every 2 hours. The original data forms a continuous time series after quality control, and the daily mean index data is obtained through calculation. The original data meets the accuracy requirements of China Meteorological Administration (CMA) and the World Meteorological Organization (WMO) for meteorological observation. Quality control includes eliminating the systematic error caused by the missing point data and sensor failure. The data is stored as an excel file.
This data set includes the observation data of 25 water net sensor network nodes in Babao River Basin in the upper reaches of Heihe River from January 2015 to December 2015. 4cm and 20cm soil moisture / temperature is the basic observation of each node; some nodes also include 10cm soil moisture / temperature, surface infrared radiation temperature, snow depth and precipitation observation. The observation frequency is 5 minutes. The data set can be used for hydrological simulation, data assimilation and remote sensing verification. For details, please refer to "2015 data document 20160501. Docx of water net of Babao River in the upper reaches of Heihe River"
The data set of bacterial diversity in Tibetan soil provides the microbial distribution characteristics of the soil surface (0-2 cm) of the Tibetan Plateau. The samples were collected from July 1st to July 15th, 2015, from three types of ecosystems: meadows, grasslands and desert. The soil samples were stored in ice packs and transported to the Ecological Laboratory of the Institute of Tibetan Plateau Research in Beijing. The DNA from the soil was extracted using an MO BIO Power Soil DNA kit. The soil surface samples were stored in liquid nitrogen after collection, shipped to the Sydney laboratory, and then extracted using a Fast Prep DNA kit. The extracted DNA samples adopted 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 909r (5'-GGACTACHVGGGTWTCTAAT-3') to amplify the 16S rRNA gene fragments. The amplified fragments were sequenced by the Illumina Miseq PE250 method, and the raw data were analyzed using Mothur software. The sequences with poor sequencing quality were first removed; the sequences were sorted, and the chimeric sequences were removed. The similarities between the sequences were then calculated, the sequences with similarities above 97% were clustered into one OTU, and the OTU representative sequence was defined. The OTU representative sequence was compared with the Silva database and identified as level one when the reliability exceeded 80%. The microbial diversities in these data on the Tibetan Plateau were systematically compared, which made them significant to the study of the microbial distribution on the Tibetan Plateau.
This data set contains the eddy correlograph observation data from January 1, 2015 to December 31, 2015 at the alou superstation, upstream of the heihe hydrometeorological observation network.The station is located in caoban village, aru township, qilian county, qinghai province.The longitude and latitude of the observation point are 100.4643e, 38.0473n and 3033m above sea level.The rack height of the vortex correlativity meter is 3.5m, the sampling frequency is 10Hz, the ultrasonic orientation is due north, and the distance between the ultrasonic wind speed and temperature meter (CSAT3) and CO2/H2O analyzer (Li7500A) is 15cm. The original observation data of the vortex correlativity instrument is 10Hz, and the published data is the 30-minute data processed by Eddypro software. The main processing steps include: outliers, delay time correction, coordinate rotation (quadratic coordinate rotation), frequency response correction, ultrasonic virtual temperature correction and density (WPL) correction.Quality assessment for each intercompared to at the same time, mainly is the atmospheric stability (Δ st) and turbulent characteristics of similarity (ITC) test.The 30min pass value output by Eddypro software was also screened :(1) data when instrument error was eliminated;(2) data of 1h before and after precipitation are excluded;(3) remove the data with a missing rate of more than 10% in the original 10Hz data within every 30 minutes;(4) the observation data of weak turbulence at night (u* less than 0.1m/s) were excluded.The average observation period was 30 minutes, 48 data per day, and the missing data was marked as -6999.Suspicious data caused by instrument drift and other reasons are marked with red font, in which the calibration data of Li7500A of the eddy current system on April 16-17 is missing;When 10Hz data is missing due to a problem with the memory card storage data (9.20-10.21,11.3-11.18), the data is replaced by the 30-min flux data output by the collector. The published observational data include:Date/Time for the Date/Time, wind Wdir (°), Wnd horizontal wind speed (m/s), standard deviation Std_Uy lateral wind speed (m/s), ultrasonic virtual temperature Tv (℃), the water vapor density H2O (g/m3), carbon dioxide concentration CO2 (mg/m3), friction velocity Ustar) (m/s), Mr. Hoff length L (m), sensible heat flux Hs (W/m2), latent heat flux LE (W/m2), carbon dioxide flux Fc (mg/(m2s)), the quality of the sensible heat flux identifier QA_Hs, the quality of the latent heat flux identifier QA_LE,Quality indicator for co2 flux QA_Fc.The quality of the sensible heat and latent heat, carbon dioxide flux identification is divided into three (quality id 0: (Δ st < 30, the ITC < 30);1: (Δ st < 100, ITC < 100);The rest is 2).The meaning of data time, such as 0:30 represents the average of 0:00-0:30;The data is stored in *.xls format. For information of hydrometeorological network or station, please refer to Liu et al. (2018), and for observation data processing, please refer to Liu et al. (2011).
This data set contains the data of meteorological element gradient observation system of alou superstation, upstream of heihe hydrometeorological observation network, from January 1, 2015 to December 31, 2015.The station is located in caoban village, aru township, qilian county, qinghai province.The longitude and latitude of the observation point are 100.4643e, 38.0473n and 3033m above sea level.The air temperature, relative humidity and wind speed sensors are located at 1m, 2m, 5m, 10m, 15m and 25m respectively, with a total of six layers facing due north.The wind direction sensor is located at 10m, facing due north;The barometer is installed at 2m;The tilting bucket rain gauge is installed on the observation tower 40m of super aru station;The four-component radiometer is installed at 5m, facing due south;Two infrared thermometers are installed at 5m, facing due south, and the probe facing vertically downward.The photosynthetic effective radiometer is installed at 5m, facing due south, and the probe facing vertically upward.Part of the soil sensor is buried at 2m in the south direction of the tower body, and the soil heat flow plate (self-correcting formal) (3 pieces) are all buried at 6cm underground.The mean soil temperature sensor TCAV is buried 2cm and 4cm underground.The soil temperature probe is buried at the surface of 0cm and underground of 2cm, 4cm, 6cm, 10cm, 15cm, 20cm, 30cm, 40cm, 60cm, 80cm, 120cm, 160cm, 200cm, 240cm, 280cm and 320cm, among which the 4cm and 10cm layers have three repeats.The soil water sensor is buried underground 2cm, 4cm, 6cm, 10cm, 15cm, 20cm, 30cm, 40cm, 60cm, 80cm, 120cm, 160cm, 200cm, 240cm, 280cm and 320cm respectively, among which the 4cm and 10cm layers have three duplexes. The observations included the following: air temperature and humidity (Ta_1 m, Ta_2 m, Ta_5 m, Ta_10 m, Ta_15 m and Ta_25 m; RH_1 m, RH_2 m, RH_5 m, RH_10 m, RH_15 m and RH_25 m) (℃ and %, respectively), wind speed (Ws_1 m, Ws_2 m, Ws_5 m, Ws_10 m, Ws_15 m and Ws_25 m) (m/s), wind direction (WD_2 m) (°), air pressure (press) (hpa), precipitation (rain) (mm), four-component radiation (DR, incoming shortwave radiation; UR, outgoing shortwave radiation; DLR_Cor, incoming longwave radiation; ULR_Cor, outgoing longwave radiation; Rn, net radiation) (W/m2), infrared temperature (IRT_1 and IRT_2) (℃), photosynthetically active radiation (PAR) (μmol/(s m-2)), average soil temperature (TCAV, ℃), soil heat flux (Gs_1, Gs_2 and Gs_3) (W/m2), soil temperature (Ts_0 cm, Ts_2 cm, Ts_4 cm_1, Ts_4 cm_2, Ts_4 cm_3, Ts_6 cm, Ts_10 cm_1, Ts_10 cm_2, Ts_10 cm_3, Ts_15 cm, Ts_20 cm, Ts_30 cm, Ts_40 cm, Ts_60 cm, Ts_80 cm, Ts_120 cm, Ts_160 cm, Ts_200 cm, Ts_240 cm, Ts_280 cm and Ts_320 cm) (℃), and soil moisture (Ms_2 cm, Ms_4 cm_1, Ms_4 cm_2, Ms_4 cm_3, Ms_6 cm, Ms_10 cm_1, Ms_10 cm_2, Ms_10 cm_3, Ms_15 cm, Ms_20 cm, Ms_30 cm, Ms_40 cm, Ms_60 cm, Ms_80 cm, Ms_120 cm, Ms_160 cm, Ms_200 cm, Ms_240 cm, Ms_280 cm and Ms_320 cm) (%, volumetric water content). Processing and quality control of observed data :(1) ensure 144 pieces of data every day (every 10min), and mark by -6999 in case of data missing;Soil temperature and humidity, soil heat flux between September 9, 2015 to September 19, 2015 and September 30, 2015 to October 20, 2015 due to power supply problems, data missing;(2) excluding the time with duplicate records;(3) data that obviously exceeds the physical significance or the range of the instrument is deleted;(4) the part marked with red letter in the data is the data in question;(5) date and time have the same format, and date and time are in the same column.For example, the time is: June 10, 2015, 10:30;(6) the naming rule is: AWS+ site name. For information of hydrometeorological network or station, please refer to Liu et al. (2018), and for observation data processing, please refer to Liu et al. (2011).