Sequencing of intestinal microorganisms of domestic animals in QTP (2019)

In order to describe the disease situation of the main domesticated animals in and around the Qinghai Tibet Plateau, the epidemic situation of the main domestic animals in the Qinghai Tibet Plateau was investigated, and the genetic samples and intestinal microbial samples of the main epidemic diseases of the main domestic animals were collected. We collected Camel dung at Mohe camel farm, Ulan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province. This data set is the 16S rRNA sequencing data of camel's intestinal microorganism in Qinghai Province. By extracting the total DNA of camel faeces, we designed primers to amplify the v3-v4 region gene fragment of bacteria, and then sequenced the corresponding data with high-throughput of the second generation. 44 samples were sequenced. This data set can be used to mine genetic resources of disease resistant individuals and find corresponding candidate genes.

0 2020-05-27

Genome resequencing data of main domestic animals in the Tibetan Plateau (2019)

In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, and to clarify the related genetic background. In 2019, we extracted total DNA from 21 local chicken tissue samples collected in Pakistan and Thailand, built a database and re sequenced the genome. Sequencing produced a batch of 140g genome re sequencing raw data. To provide basic data for the study of the adaptation of domestic animals to the extreme environment of the Qinghai Tibet Plateau, to explore the historical events of domestication, migration and expansion of the main domesticated animals in the region, and to further explore the adaptation mechanism of domesticated animals to the poor environment such as hypoxia, high cold and dry.

0 2020-05-25

Transcriptome sequencing data of main domestic animals in the Tibetan Plateau (2019)

In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, and to clarify the related genetic background. In 2019, we selected two breeds of pigs in Yunnan Province as low altitude reference, collected RNA tissue samples from the brain of two breeds of pigs, extracted total RNA, built a database and sequenced the transcriptome. Sequencing produced a batch of 322g transcriptome sequencing raw data. To provide basic data for the study of the adaptation of domestic animals to the extreme environment of the Qinghai Tibet Plateau, to explore the historical events of domestication, migration and expansion of the main domesticated animals in the region, and to further explore the adaptation mechanism of domesticated animals to the poor environment such as hypoxia, high cold and dry.

0 2020-05-25

Sequence data of Tibetan Pig transcriptome (2019)

This data set is the transcriptome data of Tibetan pigs, which are the control group and the experimental group respectively. There are three individuals in the control group without any treatment. The experimental group is also three individuals. They are attacked with FMDV at the concentration of ID50. All samples are the transcriptome sequence results of the spleen samples of Tibetan pigs. Both the experiment and sample collection were carried out in Lanzhou. The numbers are z1-z6, and each data is divided into R1 and R2, indicating the results of double headed sequencing. Z1-Z3 is the individual results of the control group, and z4-z6 is the individual results of the experimental group. By comparing and analyzing the data of the control group and the experimental group, we can find out the response of the immune system in vivo when FMDV attacks the body of Tibetan pigs, find the immune genes and immune pathways that are activated when FMDV is attacked, find the related genes and pathways for the ability of resistance of Tibetan pigs to FMDV, and increase the immunity to FMDV in the future breeding process of domestic pigs Epidemic ability provides theoretical basis.

0 2020-05-25

Plant genome re sequencing data in QTP (2019)

In order to study the relationship between the spread of cyanine and human activities, we will resequence the cyanine varieties from the Qinghai Tibet Plateau and its surrounding areas, as well as Pakistan, India, Nepal, Germany, Japan and other places. At the same time, we will cluster the gene families, and make statistics of unique, shared genes and gene families. In addition, we will also carry out the analysis of gene family expansion and contraction, and system development Tree construction, genome-wide replication events and other analysis. The aim is to analyze the molecular basis of adaptation of traditional species of cyanine to the plateau under the dual pressures of human activities and regional climate. Therefore, this study is helpful to reveal the adaptive mechanism of cyanine to adapt to the plateau ecological environment and the influence of artificial domestication and human selection on its genetic differentiation in the process of evolution.

0 2020-05-12